cdna tick samples (GenScript corporation)
Structured Review

Cdna Tick Samples, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna tick samples/product/GenScript corporation
Average 90 stars, based on 1 article reviews
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1) Product Images from "Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns"
Article Title: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns
Journal: BMC Genetics
doi: 10.1186/s12863-019-0754-8
Figure Legend Snippet: Primers sequences used to amplify the Bm86 cDNA by rt-PCR
Techniques Used: Sequencing
Figure Legend Snippet: Genetic variability of the Bm86 gene in the different sampled localities
Techniques Used:
Figure Legend Snippet: Haplogroups for sequence Bm86 from Mexican R. microplus
Techniques Used: Sequencing
Figure Legend Snippet: Mantel test, scatter plot showing correlation between genetic and geographic distances of the Bm86 haplotypes from ticks collected in different areas of the Mexican Republic. Real values for matrices are observed. a , gene distances in percentages of similarity; b , geographical distances in kilometers
Techniques Used:
Figure Legend Snippet: Minimum expansion network . It shows the frequency and relationship among haplotypes and haplogroups. Haplotypes and haplogroups are indicated with a colored label. Nine haplogroups can be observed. Haplogroups 1 and 2, containing the most abundant haplotypes. Haplotypes 10 to 64 are unique and have a random distribution in the network. GenBank Bm86 sequences included in the network come from Thailand (TH), Argentina (ARG), Mozambique (MOZ), United States of America (USA, TX), Mexico (MX), Brazil (BRA) and Australia (vaccine strain Yeerongpilly; YEERO)
Techniques Used:
Figure Legend Snippet: Molecular Phylogenetic analysis by Maximum Likelihood method. Tree reconstruction of evolutionary distances among putative sequences of the Bm86 gene of Mexican tick populations was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model. A discrete Gamma distribution was used to model evolutionary rate differences among sites (2 categories (+G, parameter = 0.0500)). Bootstrap evaluation adjusted for 1000 permutation repetitions. The main clades considered here are shown in brackets marked as A and B. Clade B has 5 subclades labeled from B1 to B5. A total of 165 complete sequences, considering amino acid residues from 20 to 627 from the Bm86 were used in the analysis, including 125 Mexican sequences (localities are marked with circles of different colors) and GenBank sequences: 14 from Texas (USA, orange triangles); 18 from Thailand (brown triangles); 1 from Australia (blue triangle); 1 from Argentina (light blue triangle); 1 from India (pink triangle); 1 from Brazil (yellow triangle); the only previous Mexican (green triangle) and 2 Mozambique sequences used as outgroup (black triangles)
Techniques Used: Labeling